1x tricine gel running buffer Search Results


complete lysis buffer  (Thermo Fisher)
99
Thermo Fisher complete lysis buffer
Complete Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complete lysis buffer - by Bioz Stars, 2026-03
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1x thermopol reaction buffer  (New England Biolabs)
97
New England Biolabs 1x thermopol reaction buffer
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Thermopol Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
1x thermopol reaction buffer - by Bioz Stars, 2026-03
97/100 stars
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1x fastbreak cell lysis buffer  (Promega)
90
Promega 1x fastbreak cell lysis buffer
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Fastbreak Cell Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
1x fastbreak cell lysis buffer - by Bioz Stars, 2026-03
90/100 stars
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1x pbs  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology 1x pbs
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Pbs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
1x pbs - by Bioz Stars, 2026-03
96/100 stars
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1x ripa buffer  (Thermo Fisher)
99
Thermo Fisher 1x ripa buffer
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Ripa Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x ripa buffer - by Bioz Stars, 2026-03
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colorless gotaq flexi buffer  (Promega)
90
Promega colorless gotaq flexi buffer
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
Colorless Gotaq Flexi Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/colorless gotaq flexi buffer/product/Promega
Average 90 stars, based on 1 article reviews
colorless gotaq flexi buffer - by Bioz Stars, 2026-03
90/100 stars
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1x pbs  (Thermo Fisher)
99
Thermo Fisher 1x pbs
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x pbs - by Bioz Stars, 2026-03
99/100 stars
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pbs  (Rockland Immunochemicals)
95
Rockland Immunochemicals pbs
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
Pbs, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
pbs - by Bioz Stars, 2026-03
95/100 stars
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1x tricane solution  (Syndel Laboratories Ltd)
90
Syndel Laboratories Ltd 1x tricane solution
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Tricane Solution, supplied by Syndel Laboratories Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
1x tricane solution - by Bioz Stars, 2026-03
90/100 stars
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1x tae buffer  (Thermo Fisher)
99
Thermo Fisher 1x tae buffer
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
1x Tae Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x tae buffer - by Bioz Stars, 2026-03
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flow cytometry staining buffer (1x)  (Bio-Techne corporation)
99
Bio-Techne corporation flow cytometry staining buffer (1x)
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
Flow Cytometry Staining Buffer (1x), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry staining buffer (1x) - by Bioz Stars, 2026-03
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flow cytometry permeabilization/wash buffer i (1x)  (Bio-Techne corporation)
96
Bio-Techne corporation flow cytometry permeabilization/wash buffer i (1x)
(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in <t>ThermoPol®</t> reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.
Flow Cytometry Permeabilization/Wash Buffer I (1x), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry permeabilization/wash buffer i (1x) - by Bioz Stars, 2026-03
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Image Search Results


(A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in ThermoPol® reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.

Journal: bioRxiv

Article Title: Multifaceted mRNA analysis using programmed RNA cleavage by Mucilaginibacter paludis Argonaute

doi: 10.1101/2025.07.01.662632

Figure Lengend Snippet: (A) pSG90 IVT RNA (∼1.7 kb) was subject to MpaAgo cleavage with single complementary DNA guides in ThermoPol® reaction buffer at 50°C for 15 minutes. The substrate:Ago molar ratio was 1:5, and the Ago:guide molar ratio was 1:5. 16 nt guides were 5’ phosphorylated and were designed for MpaAgo to cleave 100 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) are indicated above the corresponding lanes. (B) Fluc IVT RNA (∼1.8 kb) was subject to MpaAgo cleavage with single complementary DNA guides. Cleavage reactions were performed under the same conditions as in (A). 16 nt guides for Fluc RNA were also 5’ phosphorylated but were designed for MpaAgo to cleave 72 nt apart from each other. Cleavage products were analyzed on a denaturing 6% TBE-urea gel stained with SYBR Gold. Guide identities and cleavage efficiencies (%) were indicated above the corresponding lanes.

Article Snippet: 200 ng of Fluc RNA (∼1.8 kb, ∼0.37 pmol), a shortened version of the pTsin saRNA (pSG90; ∼1.7 kb, ∼0.37 pmol), or Epo RNA with a modified sequence (pSG95; ∼0.7 kb, ∼0.86 pmol), all synthesized using in vitro transcription (IVT) (New England Biolabs, #E2040) according to the manufacturer’s instructions, were cleaved with MpaAgo in a 10 μL reaction at 50°C for either 15 minutes (single and double cleavages, 1:5 substrate: Ago molar ratio) or 30 minutes (multiplexing, 1:10 substrate: Ago ratio) in 1x ThermoPol® reaction buffer (New England Biolabs #B9004S).

Techniques: Staining